bmsc human cell line hs 5  (ATCC)

Journal: Cancer Management and Research

Article Title: DCZ0814 induces apoptosis and G0/G1 phase cell cycle arrest in myeloma by dual inhibition of mTORC1/2

doi: 10.2147/CMAR.S194202

Figure Lengend Snippet: DCZ0814 induced cytotoxicity in MM cells and overcome the protective effect of the BM microenvironment. ( A ) Molecular structure of DCZ0814. ( B ) MM cells treated with DCZ0814 for 48 h and cell viability determined by CCK-8 assay. ( C ) MM cells treated with DCZ0814 for the indicated time and cell viability determined by CCK-8 assay. ( D and E ) Cell viability of ARP1 and OCI-MY5 cells treated with DCZ0814 alone or in the presence of IL-6 (25 ng/mL), IGF-1 (25 ng/mL) or BMSCs (HS-5) for 48 h. * p <0.05 compared with the 0 μM group, & p <0.05. ( F ) The percentage of Annexin-V positive cells. * p <0.05 compared with the 0 μM group. ( G ) BMSCs, ARP1, or both ARP1 and BMSCs cells were treated with DCZ0814 for 48 h, and apoptosis was detected by Annexin-V/PI staining followed by flow cytometry. Green represents BMSCs HS-5 cells, and red represents ARP1 cells. In each case, data are presented as the mean ± SD of triplicate treatments. Abbreviations: MM, multiple myeoma; BM, bone marrow; CCK-8, cell counting kit-8; IL-6, interleukin-6; IGF-1, insulin-like growth factor-1; BMSC, bone marrow stromal cell.

Article Snippet: NCI-H929, OPM2, WIL2-S and the bone marrow stroma cell (BMSC) line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: CCK-8 Assay, Staining, Flow Cytometry, Cell Counting